The most common toll paid by passenger vehicles will go from 75 cents to 95 cents, while a typical truck's tolls will be $7.85, up from $6.25.
The turnpike's annual revenues will climb from $619 million to about $738 million. The money helps subsidize 74 mass transit agencies and repair the state's bridges and roads.
The only exceptions are some newly opened sections of the turnpike in western Pennsylvania.
It will be the sixth rate change in 68 years, but the turnpike expects to begin increasing tolls every year, by about 3 percent annually.