In papers filed in Manhattan Supreme Court, the women claim they received incorrect results from the $275 Baby Gender Mentor test by Acu-Gen Biolab Inc., of Lowell, Mass.
The suit says the test maker advertised its product as the "gold standard for prenatal gender detection."
The company's Web site says the test can determine a baby's sex as early as five to eight weeks' gestation.
Lawyer Barry Gainey says the lawsuit charges the product's makers and marketers with negligence and fraud, and seeks unspecified damages.
Officials at Acu-Gen Biolab Inc. could not be reached for comment Monday night.
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