Director Chris Weitz was joined by Robert Pattinson, Taylor Lautner, Kristen Stewart and Ashley Greene.
High-pitched screams drowned out the introductions.
Those same shrill screams (of joy) also threatened to overwhelm the dialogue during the clip filmmakers showed.
The clip shows Jacob (Lautner) teaching Bella Swan (Stewart) how to ride a motorcycle, but she's distracted by visions of the vampire Edward (Pattinson) with whom she fell in love. She crashes, and Jacob takes off his shirt to soothe her wound, provoking more fangirl screams. Lautner famously gained more than 20 pounds of muscle since for the role.
Pattinson, who was greeted with adoring screams when made his Comic Con debut with "Twilight" last year, said the event was "an eye-opener, and it's just gotten bigger and bigger since."
"None of us saw it coming," Lautner added.
"It's a little overwhelming to have so many people here," Stewart said, "but I guess it's a good thing."
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