But the city has said it is nowhere near being able to enforce the law, partly due to budget problems. Under the law, unlicensed guides could be fined $300.
U.S. District Judge Jan Dubois says in the ruling Thursday that the matter is not ripe for review and that that there is no immediate threat to the tour guides.
Robert McNamara, an attorney for the three tour guides, says he was disappointed by the ruling and plans to appeal.
Other cities test and license tour guides, including Washington, New Orleans and Savannah, Ga.
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