The rapper name-checks the Steelers' QB for an incident in which he was accused by a woman of sexual assault last month.
The lyric goes: "I'd rather turn this club into a bar room brawl. Get as rowdy as Roethlisberger in a bathroom stall." Roethlisberger has been suspended for six games by NFL commissioner Roger Goodell.
The song won't be on Eminem's new album, "Recovery" - but was posted on a website that was co-founded by the rapper's manager.