The five-time Grammy award winning singer will be making an encore at the NFL championship. She was part of the halftime show during the 2000 Super Bowl.
The NFL and Fox made the announcement Monday.
Other stars to have sung the national anthem at the Super Bowl include Carrie Underwood, Billy Joel, Diana Ross, Neil Diamond, Mariah Carey and Beyonce Knowles.
The previously announced halftime show will feature The Black Eyed Peas.
The Pittsburgh Steelers will play the Green Bay Packers at Cowboys Stadium for the NFL title.