Jeter lined the first pitch he saw from Tampa Bay's Jeff Niemann to left-center for a first-inning double.
With Yankee Stadium fans standing, Jeter bounced out to third base with runners on second and third to end the second.
In the fifth, third baseman Sean Rodriguez made a sliding stop on his knees and a strong throw.
Cameras flashed as Jeter grounded to shortstop to end the seventh. He grounded again to third for the final out in the Yankees' 5-1 loss.
Jeter is on deck to become the 28th player in major league history to get 3,000 hits.