The club announced the changes after its season ended with a 3-1 loss at the Washington Nationals on Wednesday. The Phillies finished 81-81 and in third place in the NL East, ending a run of five consecutive division titles - and nine winning seasons in a row.
Perlozzo, former manager of the Baltimore Orioles, joined the Phillies as their third base coach for the 2009 season. He moved to first base in 2011.
Gross was Philadelphia's bench coach in 2001, then hitting coach from 2002-04 and from July 2010 until Wednesday.
Mackanin joined the Phillies in 2009.