Lee has agreed on a friendly wager with Baltimore Mayor Stephanie Rawlings-Blake on the outcome of the big game in New Orleans.
As part of the bet, the winning mayor will host the losing mayor to take part in a day of service with local programs from AmeriCorps, a federal community service agency.
In addition to serving the community, the mayors are putting hometown seafood on the table.
The losing mayor will visit a local crab market while wearing the jersey of the winning team and crack open the local crustacean -- either a Dungeness crab in San Francisco or a blue crab in Baltimore.
While the two mayors go head-to-head, the coaches of the Super Bowl-bound teams are facing a sibling rivalry with 49ers coach Jim Harbaugh taking on his older brother John.
Some are calling the match-up the "Har-bowl," but no matter how much competition will be on the field, both mayors are urging city residents to keep celebrations respectful and safe back at home.
Showing his support for the 49ers, Lee has declared this week "Red and Gold Week," encouraging local businesses and fans to show off their hometown pride.
During the team's playoff run, city buildings have been emanating a red and gold glow, including Coit Tower and City Hall, where the 49ers flag is also waving from the mayor's balcony.
This is the sixth time the 49ers have played in the Super Bowl, but is their first appearance since 1995.
Kickoff on Sunday is scheduled for 3:30 p.m. PST.