The Georgia State Patrol says the wreck happened around 5 a.m. Thursday on state Highway 32, near the small town of Ocilla in south Georgia.
Authorities say the Coffee County ambulance was traveling with its lights and siren activated when the semi jack-knifed across the centerline and into the path of the ambulance. The ambulance struck the left side of the semi.
The State Patrol says the patient was 65-year-old Charles Arvin Smith of Tifton. The emergency medical technicians were identified as 44-year-old Teresa Ann Davis of Axson, who was driving the ambulance, and 56-year-old Randall Whiddon of Ashburn.