Raymond Jenkins, 66, and Caroline Jenkins, 65, were identified as the victims on Wednesday afternoon.
Authorities say there were no working smoke detectors in the home.
"I saw them bring out Carol, they were doing chest compressions on her. I know they took her to St. Mary's and I know that one of her daughters followed her over there. Then I heard this morning that she didn't make it," said neighbor Geri Tomasko. The fire was reported at 8:20 p.m. Tuesday in the 300 block of Elmwood Avenue near Periwinkle Avenue.
Video from Chopper 6 showed flames shooting through the roof of the two-story, single-family farm house.
Investigators say the fire started in the basement of the home and progressed from there.
The cause of the fire remains under investigation.
It took fire crews about 30 minutes to bring the blaze under control.
Investigators were back out on Wednesday, searching through the rubble and looking for answers.
Neighbors say the victims were parents and grandparents and they are still trying to come to terms with what happened.
"When she ventured out she was always kind and she was always gentle, always had a smile on her face. I just can't believe it," said Tomasko.