The Hoyas were making their way to Philadelphia when their bus was involved in an accident on I-95 near Baltimore.
The accident involved another vehicle - an SUV with a mother and son inside, according to Georgetown Athletics.
Players and coaches, along with police officers, assisted the two from the overturned vehicle.
They were taken to the hospital as a precaution.
Georgetown University says there were no major injuries to members of the team.
Another bus was provided for the Hoyas to continue their trip.
The game between Villanova and Georgetown takes place at 7 p.m.
Full Statement from Georgetown Hoyas:
The Georgetown University men's basketball team's bus was involved in a traffic accident on Monday while traveling to Pennsylvania for a game on Tuesday. The accident involved another vehicle and occurred on I-95 North outside of Baltimore. There were no major injuries to members of the team. The company provided another bus for the team to continue its trip.