ESPN posted video on YouTube from the show "Eli's Places."
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Manning donned a long-haired wig, a mustache and prosthetics to play the part of "Chad Powers," a homeschooled freshman trying out to be a Nittany Lion.
No one seemed impressed until the undercover, two-time Super Bowl champ started throwing pigskins.
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In the end, Coach James Franklin revealed Manning to the other prospects and said "Chad Powers" failed to make the team.
Season 2 of "Eli's Places" premiered Aug. 10 on ESPN+.