Officials say a juror was dismissed on Friday afternoon, and two alternates remaining had medical issues preventing them from coming to court.
Richard Nicoletti faces charges including simple assault and reckless endangerment for his actions on June 1, 2020.
On that day, he was caught on video pepper-spraying protesters in the face on I-676, during the protests that followed George Floyd's murder.
Philadelphia protesters gassed, maced on I-676 taking legal action
Weeks after the incident, Nicoletti was fired from the police force and charges were brought against him by Philadelphia District Attorney Larry Krasner.
In 2021, the charges were dismissed in court. The judge stated that the prosecution failed to prove Nicoletti's actions were criminal.
That ruling was overturned and Nicoletti was brought to court for a second time.
READ | Charges refiled against former officer seen on video pepper-spraying protesters
Prosecutors argue that Nicoletti overstepped his position and put civilians at risk.
However, Nicoletti's defense argues that he was following orders and clearing the highway before things got out of control.
The jury told the judge it was at an impasse on Friday, but the judge urged them to come to a unanimous decision and continue working toward a verdict.
The trial and deliberations will resume Monday. Both the defense and prosecution have agreed to proceed with 11 jurors instead of 12.