All patrons will need to show proof of vaccination or a recent negative COVID-19 test to enter restaurants, bars, museums, theaters and other establishments.
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The rules take effect on Sept. 13, aimed at helping the city beat back a surge in cases from the highly contagious delta variant.
Children under the age of 12 will be exempt.
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Businesses that don't comply will be in jeopardy of being shut down or fined.
Other major U.S. cities such as San Francisco and New York have implemented similar requirements.