Crews responded to Sharp Packaging Solutions at 7451 Keebler Way around noon for a report of a chemical odor and multiple employees feeling ill.
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When emergency services arrived, they were informed that several employees were experiencing nausea, vomiting and headaches.
As a result of these symptoms, 54 employees were transported to local hospitals for evaluation, police said.
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The building has 154 employees.
Fire personnel and Lehigh County Special Operations found no abnormal readings during their investigation.
The cause of the odor has not yet been determined, police said.