The owner of the lot, Reuben Waller, is a self-described hoarder.
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Waller keeps more than 150 vehicles on the 3700 block of Old York Road.
L&I and Philadelphia police removed more than 100 vehicles from the lot earlier this month.
Waller faced a court order from the city last year to remove the collection and is facing steep fines for refusing to do so.
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Waller claims buying up the cars is a hobby and that he is not doing anything illegal.
L&I Commissioner David Perri tells Action News the owner's story is not accurate. Perri says the lot is a nuisance property, and enforcement action against it began in 2016.