The crash forced the closure of the northbound lanes for a large part of the day, but the lanes have since reopened.
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The crash happened in a construction zone of the turnpike around 6 a.m. near Mill Race Road between Quakertown (Exit 44) and Lehigh Valley (Exit 56).
The crash involved a state trooper's vehicle, a tractor-trailer and a third vehicle.
Video from a viewer shows the tractor-trailer after it burst into flames along the roadway.
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Authorities say a Pennsylvania State Police vehicle was parked in the right lane of a construction zone for safety patrol.
A crash truck equipped with an attenuator and directional sign was parked behind the state police vehicle.
Investigators say the driver of the tractor-trailer hit the attenuator, then hit the passenger side of the state police vehicle.
The truck then struck a directional sign board before finally stopping on the right shoulder of the highway where it caught fire.
Only minor injuries were reported.