It happened on Wednesday afternoon around 4 p.m. at Major League Cuts located on the unit block of South 51st Street.
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Police say a man in his 20s was rushed to an area hospital as a result of the shooting. There was no immediate word on his condition.
Chopper 6 was overhead as police officers closed off an area of the barbershop.
No other injuries were reported.
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It's unclear if any arrests were made.
Anyone with any information is asked to call police at 215-686-TIPS.