It happened around 3:30 a.m. Friday on the southbound lanes near Enterprise Avenue.
Chopper 6 over the accident site showed one vehicle crashed into the barrier.
Both vehicles were towed from the scene, with the second car being moved around 6:30 a.m.
Police said two people were injured and taken to the hospital. Their conditions were not released.
Traffic was backed up to the Walt Whitman Bridge.
I-95 reopened shortly before 7 a.m.