The crash happened around 10:30 a.m. Wednesday at the intersection of Route 206 and Jacksonville Jobstown Road.
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According to the Burlington County Prosecutor's Office, a man driving the white Jeep was going southbound on Route 206 and veered off the road into the grass.
When the Jeep came back onto the road at the intersection, it struck a Nissan, which came to rest in a nearby field. It also struck the tractor-trailer, which officials say was waiting to turn at the intersection.
The woman driving the Nissan was flown to Capital Health in Trenton. Witnesses nearby said they saw responders cut the vehicle open to get her out.
The driver of the Jeep was flown to Cooper University Hospital in Camden.
That white Jeep, which was left flipped upside down, has New Jersey state government plates.
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Officials say the driver of the tractor-trailer did not go to the hospital.
An extensive investigation followed and the intersection and surrounding roads were closed for hours as detectives worked.
The conditions of the people who were injured are not yet known.
An investigation into the cause of this crash continues.