The historic destination, often described as a Philadelphia cultural landmark, previously held the title in 2022.
Vendors and longtime businesses inside the market welcomed the news.
"It's fantastic. It definitely deserves it. The food is like no other as far as a market goes," said Jake Riehl of Riehl Deli and Cheese Shop.
To mark the win, a flash mob performed in the center food court, and the Famous 4th Street Cookie Company handed out fresh treats. Market leaders noted the award's significance because it is chosen by people who have made memories at what is recognized as the nation's oldest public market.
For the past two years, the top spot went to the Milwaukee Public Market. But with Philadelphia preparing to serve as a focal point for America 250 celebrations, voters returned the honor to Reading Terminal.
"I'm thrilled and proud to say, hey USA Today, you know what we all know, right now, so we're excited about that," Mayor Cherelle Parker said.
Beth Esposito, chef and owner of Chef Beth's Kitchen, said the recognition reflects the market's deep roots in the city.
"It's a focal point of the Philadelphia culture, and it's amazing we can be a part of it, especially at the 250th anniversary of America," she said.
Since the category's creation in 2021, Reading Terminal Market has appeared on the Top 10 list every year and has now earned the top honor twice before this year's win, making this its third overall.