The accident happened around 4:30 a.m. Saturday in the southbound lanes of Route 295 in Cherry Hill.
Police say her car slammed into the back of the parked big rig. The truck was sitting in the shoulder of the freeway.
It took rescue crews nearly a half hour to free the woman from the wreckage.
She was rushed to Cooper University Hospital, where she later died from her injuries.
The crash remains under investigation.