The crash happened near the 12th Street exit at about 7:42 a.m. Tuesday.
According to officials, the driver of one of the tractor-trailers was taken to an area hospital with non-life threatening injuries.
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As a result of the collision, the overpass for 12th Street was being inspected for damage and the road was closed at the I-95/495 split.
All northbound traffic is currently being routed onto I-95 in the construction zone. Major delays are expected.
The road closure was expected to be "lengthy," officials said. There was no estimated time for reopening.
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Drivers should seek an alternate route.