And they're making history in the progress.
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The Quakers got in one last workout Wednesday at home before departing for Alabama.
This will be their first trip to the NCAA tournament since 1995.
In all, this will be Penn's sixth Regionals appearance (1975, 1988, 1989, 1990, 1995).
Penn will face Auburn in the Auburn Regional Friday night.
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The Quakers are not short on confidence.
"We feel we're talented enough and can compete with anybody at this level honestly," said UPenn pitcher David Shoemaker.
"Hungry dogs run faster. And that's the motto the Eagles had when they went on their Super Bowl run, so we're definitely kind of feeling that right now," added second baseman Cole Palis.
Also heading to the NCAA baseball postseason, the Broncs of Rider University. The team from Lawrenceville, New Jersey qualified for its second NCAA regional appearance in the last three years by winning the MAAC conference championship last week.
Rider faces Coastal Carolina in Conway, South Carolina for the opening game on Friday night.