The sanctuary is about 20 miles northwest of West Palm Beach, not far from "Tarzan" actor Steve Sipek's home, where a 600-pound tiger named Bobo escaped in 2004. A wildlife officer shot and killed it, causing a public outcry.
A person who answered the phone at the McCarthy's Wildlife Sanctuary on Wednesday declined comment.
A lion also escaped overnight from the sanctuary, which houses about 90 animals, including six tigers, five cougars and five leopards. It was quickly captured.
Officials are looking into several possible factors, including high winds from Tropical Storm Fay, that might have allowed the animals to escape.