Harpo Productions says Winfrey will host a live episode of "The Oprah Winfrey Show" from the Kennedy Center Opera House on January 19, the day before Barack Obama takes the presidential oath of office.
Winfrey has been a prominent Obama supporter, appearing with him at campaign rallies, donating money and cheering him at the Democratic National Convention.
She famously shed tears on a stranger's shoulder in Chicago's Grant Park after Obama's election-night acceptance speech.
A Harpo spokesman says at least two shows are planned in Washington that week, the second on Wednesday.
Information about ticketing for the show will be available next week on Oprah.com.