Chidi Benjamin Uzomah Jr. entered the plea Tuesday through an attorney. A court commissioner ordered Uzomah to stay 500 yards away from Seacrest if he is released from jail on $150,000 bail.
Los Angeles police arrested Uzomah at the E! Entertainment Television headquarters in Los Angeles on Friday. A request for a civil restraining order stated he was carrying a knife at the time.
Uzomah appeared in street clothes during Tuesday's hearing and agreed to be represented by a public defender. He is due back in court Nov. 16.
Seacrest did not attend.