The country music titans will release their new album, "Nothing Like This," on Big Machine Records on Nov. 16.
Big Machine also is the home of Taylor Swift, giving the independent label two of country music's most bankable acts.
The Disney Music Group shuttered the band's only previous label, Lyric Street, in April.
Bassist Jay DeMarcus called the new partnership with Big Machine "a perfect fit" and says label president and CEO Scott Borchetta understands what the band is trying to accomplish. Disney will retain Rascal Flatts' back catalog.
The band will immediately release its latest single, "Why Wait," to country radio stations.