The Charlton Public Library's trustees this week unanimously voted to return the book to circulation, reversing the board's 1906 decision to ban the 1905 short story.
Trustee Richard Whitehead said the move was made to coincide with the American Library Association's Banned Books Week.
The book was written from the perspective of the biblical Eve. It was banned because trustee Frank Wakefield objected to nude illustrations of Eve. Whitehead tells The Telegram and Gazette he considers the illustrations works of art.
The 1906 decision drew attention from The New York Times, which reported that Twain was not particularly concerned.
Charlton is 40 miles southwest of Boston.