The crash was reported just after 1:30 p.m. on the turnpike at Route 1 in Bensalem.
The crash, involving the tractor trailer and at least one other vehicle, caused the truck to turnover onto its side.
PHOTOS: Overturned truck blocks Pa. Turnpike
Chopper 6 was over the scene where two other tractor trailers were stopped ahead of the overturned truck. It is not yet clear if they were involved in the incident.
At least one person was seriously injured, and transported to Temple University Hospital for treatment.