It happened before 6:30 a.m. Wednesday in the southbound lanes leading from the Trenton-Morrisville Bridge, which spans the Delaware River.
Images from Chopper 6 HD showed a W B Services tractor-trailer stopped across two E-ZPass lanes underneath the roof of the toll plaza.
The big rig appeared to have jumped the median dividing the two E-ZPass lanes. The cab of the truck was resting against one of the toll booths.
There were no immediate reports of injuries, or what led to the crash.
The crash was not affecting three other lanes of southbound traffic, which were proceeding through the toll plaza normally.
Pennsylvania state police and PennDOT personnel were on the scene.
Stay with Action News and 6abc.com for more information as it becomes available.