The investigation happened over the weekend following a complaint of suspicious activity at a home in Seaford, Sussex County.
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Troopers say they found several people actively engaging in dog fighting.
Five men were taken into custody and charged.
The suspects have been identified as: Byron Briddell, Kevin Land, Glenn White, Timothy Whaley and Samuel Foreman.
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Officials also rescued 14 dogs from the home. Some of the animals will be available for adoption by this weekend.
Anyone looking to help is asked to call the Brandywine Valley SPCA.