Weiner spoke to reporters Wednesday in the Capitol. One day after refusing to answer reporters' questions about the incident, he did a series of interviews in hopes of quelling the controversy over the case and insisted he was the victim of a prank.
He said: "We know for sure I didn't send this photograph."
He also couldn't resist several double entendres, saying the situation "didn't rise" to a federal investigation and that maybe the alleged hacking was "the point of al-Qaida's sword."
The photo was posted on Friday and sent to a female college student in Seattle. It was quickly deleted, but a frenzy of coverage and comment ensued on blogs, cable news outlets and other media.
The colorful and sometimes combative congressman who represents parts of Brooklyn and Queens was characteristically sharp-tongued on Tuesday, refusing to answer reporters' questions, and even calling one a "jackass" for interrupting him. He insisted that he wanted to move on from the incident, which was distracting from his work on Capitol Hill.
But that changed Wednesday, as it became clear that the story and speculation about who may have been responsible for tweeting the photo, wasn't fading.
Weiner failed in a 2005 bid for the Democratic nomination for mayor of New York City, but he is still is widely considered a future contender for the office.
Weiner was a Twitter follower of the female college student who received the photo.
The tweet of the lewd photo first was reported Saturday by BigGovernment.com, a website run by conservative commentator Andrew Breitbart.
The site said the photo was tweeted to a female college student in Seattle. The woman told the New York Daily News that she had never met Weiner, and that there had "never been any inappropriate exchanges" between her and the congressman.
Weiner, 46, is married to Huma Abedin, an aide to Secretary of State Hillary Clinton.
The congressman over the weekend joked about the incident on Twitter, asking whether his kitchen blender would be next to "attack" him.
On Tuesday as the House was preparing to vote on the nation's debt limit, Weiner was back tweeting about the vote with his usual partisan edge.
