Alyssa, Lauren and Rebecca Barlow are releasing their final song, "Hope Will Lead Us On," on Oct. 30. They will hold an online chat with fans Oct. 29 on Ustream, where they will take questions and perform acoustically.
No reason was given for the decision to retire. A spokesman says, simply, "They're ready for a new season."
In a statement, the sisters call their music career "one of the most incredible adventures." They thanked fans for sharing their dream.
Barlowgirl has sold over a million copies of their self-titled debut album released in 2004. They earned four No. 1 hits, including 2005's "I Need You To Love Me" which spent a record 13 weeks at the top.