The accident happened at the Pennsylvania/Delaware state line, just before the split for I-495 and I-95 southbound in Boothwyn, Pennsylvania.
State police say a car cut the tractor-trailer off, causing the truck to swerve and crash into a nearby guardrail.
The trailer the truck was hauling was empty, but more than 100 gallons of fuel leaked from the truck's fuel tanks, police say.
No injuries were reported.
Interstate 495 southbound lanes were shut down, with only one lane to I-95 southbound getting by as crews worked to clean up the scene.
All lanes have since been reopened.