"Because I was not picked by the French federation to compete for a medal in singles, and because I need to maintain my fitness ... I have decided not to take part in the Beijing Games to concentrate on my last major objective of the season - the U.S. Open," Mauresmo said on her Web site.
Mauresmo, now ranked No. 33, said she reached the decision "with sadness, because competing for my country in my last Olympics was very dear to my heart."
At Wimbledon, which she won in 2006, Mauresmo lost to Serena Williams in the third round.