Many guests at Rosie's Place, a Boston shelter, have been victims of violence, public relations director Leemarie Mosca said.
"In light of our mission, we don't think violence against women is a laughing matter," she said.
Mosca said the shelter expected to book a replacement before the Oct. 16 luncheon "Funny Women ... Serious Business."
"Right now, our main focus is our event and making sure the event is a success," Mosca said. "And for us, that means not including Sandra Bernhard at this time."
A representative for Bernhard had no immediate comment on the cancellation.
In a statement on her Web site, Bernhard said the gang-rape comment was part of a larger piece from her show about "racism, freedom, women's rights, and the extreme views of Gov. Sarah Palin - a woman who doesn't believe that other women should have the right to choose."
"I certainly wish Gov. Palin no harm - I'd just like her to explain to me how she can hold such outrageous views ... and then go back to Alaska," she said.