Robert Pattinson, Kristen Stewart and Taylor Lautner will appear together on the show - and see what they could look like with a Jersey-style makeover.
Kimmel has taped segments with the cast of MTV's "Jersey Shore" spoofing "Twilight."
There's Mike "The Situation" Sorrentino dressed like Cullen but shirtless in a tanning bed coffin and Nicole "Snooki" Polizzi as Bella Swan wearing a yellow minidress. Lautner's character of werewolf Jacob Black is played by Pauly "DJ Pauly D" Del Vecchio.
Kimmel plays Forks police chief and Bella's father, Charlie.
"Jimmy Kimmel Live's Twilight Saga: Total Eclipse of the Heart" airs Wednesday, June 23 at 10 p.m. ET/PT on ABC.
"Eclipse" opens June 30.