A car plowed into the ambulance and burst into flames on Broad Street at 66th Avenue in East Oak Lane around 2:45 a.m.
The driver of the car was killed.
Police say a 70-year-old patient and another man in the back of the ambulance were injured in the wreck.
Those men, and the 24-year-old medic driving the ambulance, are being treated at nearby hospitals.
The ambulance driver suffered a broken femur but is expected to be okay.
Investigators haven't said what caused the tragedy.