The hackers said Friday that they were responsible for defacing the website of the Dayton, Ohio-based chapter of Infragard, a public-private partnership for critical infrastructure protection sponsored by the FBI.
The Ohio site was replaced with the video for Coolio's 1995 rap hit and a profane message attacking Infragard as a "sinister alliance" between corporations and law enforcement.
Anonymous has promised weekly hacks as the amorphous group continues its campaign against law enforcement worldwide.
The FBI did not immediately return a call seeking comment.