It happened around 2:30 a.m. Sunday as the party in the 700 block of Russell Street was winding down.
Police say two men started arguing when one pulled out a gun and started firing at the other.
A 26-year-old man was hit and is in critical condition at Temple University Hospital.
A 26-year-old woman and a 54-year-old man were struck by stray bullets. They are in stable condition at Temple.
No arrests have been made.