New York State Lottery officials say the ticket was sold in Suffolk County, at a store in Rocky Point called Cards and Things.
No one has yet claimed the prize from Tuesday night's drawing in the multistate lottery.
The winning numbers were 3, 33, 35, 39, 45 and Mega Ball number 13.
Lottery spokeswoman Jennifer Givner says the ticket shows a lump-sum option, meaning the cash would be paid out right away instead of $162 million over 26 years.
But there's a catch: The prize then shrinks to about $100 million.
Mega Millions tickets are sold in Georgia, Illinois, California, Texas, Washington, Virginia, Ohio, New York, New Jersey, Michigan, Maryland and Massachusetts.