The crash happened shortly before 1:30 p.m. Wednesday in the eastbound lanes of I-78 in Greenwich Township, Berks County.
State police troopers say the tractor trailer driver died on the scene a short time after the crash.
PennDOT reported the crash closed the eastbound lanes of I-78 between Route 737 in Krumsville and Route 863 in New Smithville, Lehigh County.