The accident happened at 9:30 a.m. Thursday in the northbound lanes of the highway several miles north of Exit 7 at mile marker 57.7 in Hamilton Township.
State police tell Action News two women were trapped in a vehicle, and several people on the bus were complaining of back pain.
Images from Chopper 6 HD over the scene showed a gray dump truck, which appeared to have rear-ended the Megabus.
A blue passenger vehicle, which had sustained heavy damage, was located a short distance away.
Crews worked for several hours to clear the scene. The turnpike was finally reopened just after 2:00 p.m.