The National Park Service has warned the protesters at McPherson Square and at Freedom Plaza that those who violate the camping rule beginning Monday at noon will be subject to arrest.
Protesters have said they intend to stay at the two sites and defend their encampments.
The enforcement comes after a House oversight subcommittee hearing last week where Republican lawmakers questioned why the park service has allowed occupy protesters to camp for months on federal land. National Park Service Director Jonathan Jarvis said protesters have a right to be in the park and won't be evicted, but they will be encouraged to sleep elsewhere.
